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Tobacco smoking results in the absorption of nicotine through the lung and buccal/nasal epithelium, after which nicotine is metabolized into about 20 metabolites excreted in urine. Cotinine, a major metabolite, accumulates in the body with regular smoking. It is reported that cotinine is stable in body fluids and has a relatively long half life of approximately 17 hours. Therefore, the detection of cotinine is less dependent on the time of sampling than that of nicotine and other metabolites.
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Smoke check test for nicotine | cotinine